[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].
Identifieur interne : 003043 ( Main/Exploration ); précédent : 003042; suivant : 003044[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].
Auteurs : Fang Huang [République populaire de Chine] ; Ying-Jun Chi ; Hui He ; De-Yue YuSource :
- Yi chuan = Hereditas [ 0253-9772 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN complémentaire (composition chimique), ADN complémentaire (génétique), Aldehyde dehydrogenase (classification), Aldehyde dehydrogenase (génétique), Analyse de profil d'expression de gènes (MeSH), Analyse de séquence d'ADN (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Feuilles de plante (génétique), Fleurs (génétique), Phylogenèse (MeSH), Protéines de soja (génétique), Protéines végétales (génétique), RT-PCR (MeSH), Racines de plante (génétique), Régulation de l'expression des gènes végétaux (MeSH), Similitude de séquences d'acides aminés (MeSH), Soja (enzymologie), Soja (génétique), Séquence d'acides aminés (MeSH).
- MESH :
- classification : Aldehyde dehydrogenase.
- composition chimique : ADN complémentaire.
- enzymologie : Soja.
- génétique : ADN complémentaire, Aldehyde dehydrogenase, Feuilles de plante, Fleurs, Protéines de soja, Protéines végétales, Racines de plante, Soja.
- Analyse de profil d'expression de gènes, Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Phylogenèse, RT-PCR, Régulation de l'expression des gènes végétaux, Similitude de séquences d'acides aminés, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Aldehyde Dehydrogenase (classification), Aldehyde Dehydrogenase (genetics), Amino Acid Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Complementary (chemistry), DNA, Complementary (genetics), Flowers (genetics), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Leaves (genetics), Plant Proteins (genetics), Plant Roots (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH), Soybean Proteins (genetics), Soybeans (enzymology), Soybeans (genetics).
- MESH :
- chemical , chemistry : DNA, Complementary.
- chemical , classification : Aldehyde Dehydrogenase.
- chemical , genetics : Aldehyde Dehydrogenase, DNA, Complementary, Plant Proteins, Soybean Proteins.
- enzymology : Soybeans.
- genetics : Flowers, Plant Leaves, Plant Roots, Soybeans.
- Amino Acid Sequence, Cloning, Molecular, Gene Expression Profiling, Gene Expression Regulation, Plant, Molecular Sequence Data, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid.
Abstract
To research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. A gene predominately expressed in soybean flowers was chosen for further analysis. Through bioinformatic and RT-PCR approaches, the full-length gene was cloned from soybean flowers. The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1. GmALDH3-1 contains a complete open reading frame of 1485 bp in length, which encodes for a peptide of 494 amino acids. The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3, respectively, and 39% and 59% with human ALDH3B. Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1. Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers, but the expression of this gene was almost undetectable in leaves and roots. We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium, epidermis, outer integument, and hilum.
DOI: 10.3724/sp.j.1005.2010.00492
PubMed: 20466639
Affiliations:
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Acid (MeSH)</term><term>Soybean Proteins (genetics)</term><term>Soybeans (enzymology)</term><term>Soybeans (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (composition chimique)</term><term>ADN complémentaire (génétique)</term><term>Aldehyde dehydrogenase (classification)</term><term>Aldehyde dehydrogenase (génétique)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Fleurs (génétique)</term><term>Phylogenèse (MeSH)</term><term>Protéines de soja (génétique)</term><term>Protéines végétales (génétique)</term><term>RT-PCR (MeSH)</term><term>Racines de plante (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Soja (enzymologie)</term><term>Soja (génétique)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Aldehyde Dehydrogenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aldehyde Dehydrogenase</term><term>DNA, Complementary</term><term>Plant Proteins</term><term>Soybean Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Aldehyde dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN complémentaire</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Soja</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="genetics" 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moléculaires</term><term>Phylogenèse</term><term>RT-PCR</term><term>Régulation de l'expression des gènes végétaux</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. A gene predominately expressed in soybean flowers was chosen for further analysis. Through bioinformatic and RT-PCR approaches, the full-length gene was cloned from soybean flowers. The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1. GmALDH3-1 contains a complete open reading frame of 1485 bp in length, which encodes for a peptide of 494 amino acids. The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3, respectively, and 39% and 59% with human ALDH3B. Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1. Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers, but the expression of this gene was almost undetectable in leaves and roots. We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium, epidermis, outer integument, and hilum.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20466639</PMID><DateCompleted><Year>2010</Year><Month>12</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0253-9772</ISSN><JournalIssue CitedMedium="Print"><Volume>32</Volume><Issue>5</Issue><PubDate><Year>2010</Year><Month>May</Month></PubDate></JournalIssue><Title>Yi chuan = Hereditas</Title><ISOAbbreviation>Yi Chuan</ISOAbbreviation></Journal><ArticleTitle>[Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean].</ArticleTitle><Pagination><MedlinePgn>492-7</MedlinePgn></Pagination><Abstract><AbstractText>To research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. A gene predominately expressed in soybean flowers was chosen for further analysis. Through bioinformatic and RT-PCR approaches, the full-length gene was cloned from soybean flowers. The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1. GmALDH3-1 contains a complete open reading frame of 1485 bp in length, which encodes for a peptide of 494 amino acids. The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3, respectively, and 39% and 59% with human ALDH3B. Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1. Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers, but the expression of this gene was almost undetectable in leaves and roots. We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium, epidermis, outer integument, and hilum.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Fang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. fhuang@njau.edu.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chi</LastName><ForeName>Ying-Jun</ForeName><Initials>YJ</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Hui</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>De-Yue</ForeName><Initials>DY</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Yi Chuan</MedlineTA><NlmUniqueID>9436478</NlmUniqueID><ISSNLinking>0253-9772</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D030262">Soybean Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.2.1.3</RegistryNumber><NameOfSubstance UI="C553864">ALDH3-1 protein, soybean</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.2.1.3</RegistryNumber><NameOfSubstance UI="D000444">Aldehyde Dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000444" MajorTopicYN="N">Aldehyde Dehydrogenase</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D035264" MajorTopicYN="N">Flowers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030262" MajorTopicYN="N">Soybean Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20466639</ArticleId><ArticleId IdType="pii">0253-9772(2010)5-492-06</ArticleId><ArticleId IdType="doi">10.3724/sp.j.1005.2010.00492</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chi, Ying Jun" sort="Chi, Ying Jun" uniqKey="Chi Y" first="Ying-Jun" last="Chi">Ying-Jun Chi</name><name sortKey="He, Hui" sort="He, Hui" uniqKey="He H" first="Hui" last="He">Hui He</name><name sortKey="Yu, De Yue" sort="Yu, De Yue" uniqKey="Yu D" first="De-Yue" last="Yu">De-Yue Yu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Fang" sort="Huang, Fang" uniqKey="Huang F" first="Fang" last="Huang">Fang Huang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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